Human WNT10A (Wingless Type MMTV Integration Site Family, Member 10A) ELISA Kit | ELK6212

Human WNT10A (Wingless Type MMTV Integration Site Family, Member 10A) ELISA Kit | ELK6212 | Gentaur UK, US & Europe Distribution

Alternative Names: FLJ14301; OODD; Protein Wnt-10a; SSPS; STHAG4; WN10A_HUMAN; wnt10a

Species: Human

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 10A (WNT10A) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 10A (WNT10A) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 10A (WNT10A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 10A (WNT10A) in the samples is then determined by comparing the OD of the samples to the standard curve.