Human XCL2 (Chemokine C-Motif Ligand 2) ELISA Kit | ELK3409

Human XCL2 (Chemokine C-Motif Ligand 2) ELISA Kit | ELK3409 | Gentaur UK, US & Europe Distribution

Alternative Names: SCYC2; SCM-1b; Small Inducible Cytokine Subfamily C Member 2; Cytokine SCM-1 beta; C motif chemokine 2; XC chemokine ligand 2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Cytokine;Signal transduction;Tumor immunity;Infection immunity;Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-Motif Ligand 2 (XCL2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chemokine C-Motif Ligand 2 (XCL2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-Motif Ligand 2 (XCL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-Motif Ligand 2 (XCL2) in the samples is then determined by comparing the OD of the samples to the standard curve.