Human XRCC5 (X-Ray Repair Cross Complementing 5) ELISA Kit | ELK4469

Human XRCC5 (X-Ray Repair Cross Complementing 5) ELISA Kit | ELK4469 | Gentaur UK, US & Europe Distribution

Alternative Names: KARP1; KU80; KUB2; Ku86; NFIV; TLAA; Lupus Ku Autoantigen Protein P80; Nuclear factor IV; 86 kDa of Ku antigen; CTC box-binding factor 85 kDa; Thyroid-lupus autoantigen

Species: Human

Assay Type: Sandwich

Sensitivity: 0.057 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to X-Ray Repair Cross Complementing 5 (XRCC5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to X-Ray Repair Cross Complementing 5 (XRCC5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain X-Ray Repair Cross Complementing 5 (XRCC5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of X-Ray Repair Cross Complementing 5 (XRCC5) in the samples is then determined by comparing the OD of the samples to the standard curve.