855
Human YWHAz (Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta) ELISA Kit | ELK7507
- SKU:
- 855-ELK7507-GEN
Description
Human YWHAz (Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta) ELISA Kit | ELK7507 | Gentaur UK, US & Europe Distribution
Alternative Names: KCIP-1; 14-3-3-zeta; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1
Species: Human
Assay Type: Sandwich
Sensitivity: 0.122 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) in the samples is then determined by comparing the OD of the samples to the standard curve.