Human ZPI (Protein Z Dependent Protease Inhibitor) ELISA Kit | ELK3286

Human ZPI (Protein Z Dependent Protease Inhibitor) ELISA Kit | ELK3286 | Gentaur UK, US & Europe Distribution

Alternative Names: PZI; SERPINA10; Serine (Cysteine) Proteinase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin) Member 10; Protein Z-Related Protease Inhibitor

Species: Human

Assay Type: Sandwich

Sensitivity: 0.49 ng/mL

Standard: 80 ng/mL

Detection Range: 1.25-80 ng/mL

Sample Type: plasma

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Z Dependent Protease Inhibitor (ZPI) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein Z Dependent Protease Inhibitor (ZPI) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Z Dependent Protease Inhibitor (ZPI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Z Dependent Protease Inhibitor (ZPI) in the samples is then determined by comparing the OD of the samples to the standard curve.