I-Ek Antibody [14-4-4S] | 99-039

I-Ek Antibody [14-4-4S] | 99-039 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Mouse, Rat

Homology: N/A

Immunogen: C3H mouse skin graft and splenocytes

Research Area: Other

Tested Application: WB, IHC-Fr, ICC, Flow, IP

Application: I-Ek Antibody [14-4-4S] for use in flow cytometry, immunohistochemistry / immunocytochemistry, immunoprecipitation, CMCD, activation, and blocking assays.

Specificiy: I-Ek|RT1D

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [14-4-4S]

Isotype: IgG2a

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added

Concentration: 0.5 mg/mL

Storage Condition: Store vial at 2-8˚C

Alternate Name: MHC Class II

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The monoclonal antibody 14-4-4S reacts with the I-Ek class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen. The antibody has been reported to cross-react with the rat MHC class II alloantigen RT1D.