ICAM-1 Antibody | 38-217

ICAM-1 Antibody | 38-217 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: N/A

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTIMP-1 (human Tissue Inhibitor of Metalloproteinases-1) . Human TIMP-1 specific antibody was purified by affinity chromatography and then biotinylated.

Research Area: Antibody Pairs, Functional Assays

Tested Application: E, WB, Neut

Application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human ICAM-1 (10 ng/mL) , a concentration of 8.0 μ/mL of this antibody is required.
ELISA:
Direct:
To detect hTIMP-1 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTIMP-1.
Sandwich
To detect hTIMP-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human TIMP-1 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTIMP-1.
Western Blot:
To detect hTIMP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTIMP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: ICAM-1 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

Alternate Name: BB2, CD54, P3.58Intercellular adhesion molecule 1, Major group rhinovirus receptor, ICAM-1

User Note: Centrifuge vial prior to opening.

BACKGROUND: N/A