ICL | Isocitrate lyase | AS09 500

ICL | Isocitrate lyase | AS09 500 | Gentaur UK, US & Europe Distribution

Immunogen: Purified native ICL from Ricinus communis

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Ammonium sulfate purified total IgG.

Format: Lyophilized

Tested Application: ELISA (ELISA), Western blot (WB)

Related Products: collection of antibodies to proteins involved in carbohydrate metabolism Plant protein extraction bufferSecondary antibodies

Recommended Dilutions: 1 : 8000 (ELISA), 1 : 2000 (WB)

Molecular weight: 64, 7 | 65 kDa

Confirmed Reactivity: Ricinus communis

Predicted Reactivity: Arabidopsis thaliana, Cucumis sativus, Gossypium mexicanum, Oryza sativa, Populus alba x tremula, Populus balsamifera subsp. trichocarpa, Sorghum bicolorSpecies of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.ICL protein is most abundant in young cotyledons in germinating seedlings, not in rosette leaves or shoots.

Background: Isocitrate lyase (EC=4.1.3.1), localized in glyoxysome is an enzyme involved in mobilization of storage lipis during plant seedling growth. Catalalyzed reaction is a conversion of isocitrate to succinate and glyoxylate. Alternative names of this enzyme: isocitrase, isocitritase, isocitratase, glyoxylate-lyase, isocitrate glyoxylatelyase.

Reconstitution: For reconstitution add 100 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT3G21720

Category: Carbohydrates

Research Area: Plant Biology