IGF-BP2, human recombinant | 7165

IGF-BPs controls the distribution, function and activity of IGFs in various cell tissues and body fluids. Currently there are seven named IGF-BPs that form high affinity complexes with both IGF-I and IGF-II. IGF-BP2 is a cysteine-rich secreted protein produced by bone cells, and is most abundant in the brain. IGF-BP2 has been shown to inhibit IGF-II action in human breast and ovarian carcinoma cells. Recombinant human IGF-BP2 is a 31.5 kDa protein consisting of 289 amino acid residues including the IGF-BP domain and thyroglobulin type-I domain.

7165 | IGF-BP2 human recombinant DataSheet

Biomolecule/Target: IGF-BP2

Synonyms: IBP-2

Alternates names: Malate dehydrogenase 2, NAD (mitochondrial), M-MDH, MDH, MOR1

Taglines: Inhibits IGF-II action in human breast and ovarian carcinoma cells

NCBI Gene ID #: 4191

NCBI Gene Symbol: MDH2

Gene Source: Human

Accession #: P40926

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): N/A

Biological activity: Specific activity is > 30 units/mg, and is defined as the amount of enzyme that cleaves 1 µmole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH 7.5 at 25°C.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 35.2 kDa (335 aa, 25-338 aa + NT His Tag)

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 1 x PBS, pH 7.2.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET KAAVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMICVIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL TALTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVKSQET ECTYFSTPLL LGKKGIEKNL GIGKVSSFEE KMISDAIPEL KASIKKGEDF VKTLK