IGF-BP6, human recombinant | 7350

IGFBP6 contains 1 IGFBP N-terminal domain and 1 thyroglobulin type-1 domain. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Recombinant human IGFPB6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

7350 | IGF-BP6 human recombinant DataSheet

Biomolecule/Target: IGFBP6

Synonyms: Insulin-like growth factor-binding protein 6, IBP6

Alternates names: Insulin-like growth factor-binding protein 6, IBP6

Taglines: Regulates the growth promoting effects of the IGFs on cell culture

NCBI Gene ID #: 77125

NCBI Gene Symbol: IL33

Gene Source: Human

Accession #: Q8BVZ5

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED as determined by the dose-dependent stimulation of the proliferation of murine D10S cells is 0.5 ng/ml, corresponding to a specific activity of 2 x 10 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 25 kDa (236 aa, 28-240 aa + His Tag), confirmed by MALDI-TOF.

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: 0.5 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 1 mM DTT and 20% glycerol.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGSRCPGCGQ GVQAGCPGGC VEEEDGGSPA EGCAEAEGCL RREGQECGVY TPNCAPGLQC HPPKDDEAPL RALLLGRGRC LPARAPAVAE ENPKESKPQA GTARPQDVNR RDQQRNPGTS TTPSQPNSAG VQDTEMGPCR RHLDSVLQQL QTEVYRGAQT LYVPNCDHRG FYRKRQCRSS QGQRRGPCWC VDRMGKSLPG SPDGNGSSSC PTGSSG