IGF-I, human recombinant | 4119

Human IGF-1 (insulin-like Growth Factor-1, IGF1, MGF, Somatomedin) is a hormone similar in molecular structure to insulin. It is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. Insulin-like growth factor 1 has been shown to bind and interact with all the IGF-1 Binding Proteins (IGFBPs), of which there are six (IGFBP1-6). IGFBP-3, the most abundant protein, accounts for 80% of all IGF binding. IGF-1 binds to IGFBP-3 in a 1:1 molar ratio. IGF-1 binds to at least two cell surface receptors: the IGF-1 receptor (IGF1R), and the insulin receptor. The IGF-1 receptor seems to be the "physiologic" receptor - it binds IGF-1 at significantly higher affinity than the IGF-1 that is bound to the insulin receptor. Mature human IGF I shares 94% and 96% aa sequence identity with mouse IGF1 and rat IGF1, respectively, and exhibits cross species activity. It shares 64% aa sequence identity with mature human IGFII. Human IGF-1 is a 7.6 kDa protein containing 70 amino acid residues. The recombinant human IGF-1 was produced using animal origin free technology.

4119 | IGF-I human recombinant DataSheet

Biomolecule/Target: IGF-I

Synonyms: Insulin-Like Growth Factor 1, Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF

Alternates names: Superoxide dismutase, manganese, MnSOD.

Taglines: Human IGF-1 from E.Coli, AOF, 98% pure, biologically active, cell culture tested

NCBI Gene ID #: N/A

NCBI Gene Symbol: N/A

Gene Source: Bacteria

Accession #: N/A

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: 98%

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: Specific activity is >350 units/mg

Results: >350 units/mg

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 25.2 kDa (226 aa, 1-206 aa + His Tag)

Concentration: N/A

Appearance: Recombinant IGF-1 is available as a lyophilized powder

Physical form description: IGF-1 protein is lyophilized with no additives

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MSYTLPSLPY AYDALEPHFD KQTMEIHHTK HHQTYVNNAN AALESLPEFA NLPVEELITK LDQLPADKKT VLRNNAGGHA NHSLFWKGLK KGTTLQGDLK AAIERDFGSV DNFKAEFEKA AASRFGSGWA WLVLKGDKLA VVSTANQDSP LMGEAISGAS GFPIMGLDVW EHAYYLKFQN RRPDYIKEFW NVVNWDEAAA RFAAKK