Additional Information
Size: |
100 µl |
Target: |
IkB epsilon (pSer22) |
Conjugate: |
RPE |
Research Area: |
Cell Signaling | Epigenetics and Nuclear Signaling | Nuclear Signaling Pathways | NFkB Pathway | Immunology | Innate Immunity | TLR Signaling | Cell Signaling | Epigenetics and Nuclear Signaling | Transcription | Transcription Factors |
Alternative Name: |
IκB-ε Antibody, IkB-epsilon Antibody, NFKBIE Antibody, IkappaBepsilon Antibody, IKBE Antibody, IkB-E Antibody, NF-kappa-BIE Antibody, I-kappa-B-epsilon Antibody, NF-kappa-B inhibitor epsilon Antibody |
Category: |
Antibodies |
Product Type: |
Polyclonal Antibody |
Immunogen: |
Synthetic phospho-peptide from human IkB epsilon around the phosphorylation site of serine 22 (pSer22). |
Immunogen Species: |
Human |
Applications: |
WB, IHC, ICC/IF, ELISA |
Host: |
Rabbit |
Isotype: |
IgG |
Species Reactivity: |
Human, Mouse, Rat |
Antibody Dilution: |
WB (1:250-1:500); IHC (1:25-1:50); ICC/IF (1:50-1:250); ELISA (1:20000); optimal dilutions for assays should be determined by the user. |
Purification: |
Peptide Affinity Purified |
Storage Buffer: |
PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide |
Concentration: |
N/A |
Specificity: |
Detects endogenous levels of IkB epsilon only when phosphorylated at serine 22 (pSer22). |
Storage: |
-20ºC |
Shipping: |
Blue Ice or 4ºC |
Certificate of Analysis: |
A 1:500 dilution of SPC-1392 was sufficient for detection of IkB epsilon (pSer22) in 10 µg of human jurkat cell lysates by ECL immunoblot analysis using goat anti-rabbit as the secondary antibody. |
Cellular Localization: |
Cytoplasm, Cytosol, Golgi Apparatus, Nucleus, Fibrillar Center, Perinuclear Region Of Cytoplasm |
Tissue Specificity: |
Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages. |
Accession Number: |
NP_004547.2 |
Gene ID: |
4794 |
Swiss-Prot: |
O00221 |
Field of Use: |
For in vitro research use only. |