IkB-α (N-terminus) monoclonal Antibody | MB0106

IkB-α (N-terminus) monoclonal Antibody | MB0106 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Application: WB

Application Range: WB: 1:1000

Background: Activation of NFκB requires that IκB be phosphorylated on specific serine residues, which results in targeted degradation of IκB. IκB kinase α (IKKα), previously designated CHUK, interacts with IκB-α and specifically phosphorylates IκB-α on the sites that trigger its degradation Serines 32 and 36. IKKα appears to be critical for NFκB activation in response to proinflammatory cytokines. Phosphorylation of IκB by IKKα is stimulated by the NFκB inducing kinase (NIK), which itself is a central regulator for NFκB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ, and each appear to make essential contributions to IκB phosphorylation.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This Antibody detects endogenous levels of IkB-alpha and does not cross-react with related proteins

Molecular Weight: Predicted band size:36KDa Observed band size:36KDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3; NFKBIA; IKBA; MAD3; NFKBI

Immunogen: Synthetic peptide, corresponding to the N-terminus of Human IkB-α.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: NF-kB Signaling,