IkB-α (phospho-Y42) polyclonal Antibody | BS4736

IkB-α (phospho-Y42) polyclonal Antibody | BS4736 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB IHC

Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200

Background: Activation of NFκB requires that IκB be phosphorylated on specific serine residues, which results in targeted degradation of IκB. IκB kinase α (IKKα), previously designated CHUK, interacts with IκB-α and specifically phosphorylates IκB-α on the sites that trigger its degradation Serines 32 and 36. IKKα appears to be critical for NFκB activation in response to proinflammatory cytokines. Phosphorylation of IκB by IKKα is stimulated by the NFκB inducing kinase (NIK), which itself is a central regulator for NFκB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ, and each appear to make essential contributions to IκB phosphorylation.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: p-IκB-α (Y42) polyclonal Antibody detects endogenous levels of IκB-α protein only when phosphorylated at Tyr42.

Molecular Weight: ~ 36 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3; NFKBIA; IKBA; MAD3; NFKBI

Immunogen: Synthetic phosphopeptide derived from human IkB-α around the phosphorylation site of Tyrosine 42.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: ErbB HER Signaling,