IKK alpha Antibody | 2025

IKK alpha Antibody | 2025 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (94%) , Mouse: (79%)

Immunogen: IKK alpha antibody was raised against a 19 amino acid peptide near the carboxy terminus of human IKK alpha.
The immunogen is located within the last 50 amino acids of IKK alpha.

Research Area: Signal Transduction

Tested Application: E, WB, ICC, IF

Application: IKK alpha can be used for detection of IKK alpha by Western blot at 1 μg/mL. An 85 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: Antibody has no cross response to IKKb or IKKg.

Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 2: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 3: Cat. No. 17-005 - Jurkat Cell Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 85 kDa

Validation:

Overexpression validation (Figure 8) : ASC expression detected by anit-ASC antibodies (2287) was shown in ASC transfected 293T cells, but not in WT 293T cells.

Isoform: N/A

Purification: IKK alpha Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: IKK alpha Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: IKK alpha antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: IKK alpha Antibody: IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase alpha

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: IKK alpha Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The long-sought IκB kinase (IKK) , which phosphorylates IκB, and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα and IKKβ) . IKKα and IKKβ interact with each other and both are essential for the NF-κB activation. IKKα specifically phosphorylates IkB-alpha. IKKα is expressed in variety of human tissues.