IKK epsilon Antibody | 2329

IKK epsilon Antibody | 2329 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Rat: (75%)

Immunogen: Anti-IKK epsilon antibody (2329) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IKK epsilon.
The immunogen is located within the last 50 amino acids of IKK epsilon.

Research Area: Signal Transduction

Tested Application: E, WB, IHC-P, IF

Application: WB: 1-2 μg/mL; IHC: 10 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: It has no cross response to IKKa, IKKb, or IKKy.

Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 2: Cat. No. 1307 - Human Pancreas Tissue Lysate

Positive Control 3: Cat. No. 11-101 - Human Pancreas Tissue Slide

Positive Control 4: Cat. No. 1202 - A431 Cell Lysate

Positive Control 5: Cat. No. 1224 - Daudi Cell Lysate

Positive Control 6: N/A

Molecular Weight: Predicted: 80kD
Observed: 80 kD

Validation:

Induced Expression Validation (Figure 1) : Raw 264.7 cells were treated with LPS (0.3 µg /mL) for different time period (3 hrs and 6 hrs) . IKK epsilon expression detected by anit-IKK epsilon antibodies (2329) was induced by LPS treatment and the expression levels were increased gradually at 3 hrs and 6 hrs.

Isoform: Human IKK epsilon has 2 isoforms, including isoform 1 (716aa, 80kD) and isoform 2 (631aa, 70kD) . Mouse IKK epsilon has one isoform (717aa, 81kD) and Rat IKK epsilon also has one isoform (717aa, 80kD) . 2329 can detect human, mouse and possibly rat.

Purification: IKK epsilon Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: IKK epsilon Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: IKK epsilon antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: IKK epsilon Antibody: IKKE, IKKI, IKK-E, IKK-i, IKKE, KIAA0151, Inhibitor of nuclear factor kappa-B kinase subunit epsilon, Inducible I kappa-B kinase, I-kappa-B kinase epsilon

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: IKK epsilon Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. IκB is phosphorylated by IκB kinase (IKK) complex that contains IKKα, IKKβ, and IKKγ. A novel molecule in the IKK complex was recently identified and designated IKKε/IKK-i. IKK epsilon is required for the activation of NF-κB by PMA and T cell receptors but not by TNFα and IL-1. IKKε/IKK-i message is expressed in a variety of tissues and is inducible by TNFα, IL-1, and LPS.