IKKβ (Y188) polyclonal Antibody | AP0639

IKKβ (Y188) polyclonal Antibody | AP0639 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB IF

Application Range: WB: 1:2000~1:5000 IF: 1:50~1:200

Background: The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IKKβ (Y188) polyclonal Antibody detects endogenous levels of IKKβ protein.

Molecular Weight: ~ 96 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta;IKK-B; IKK-beta; IkBKB;I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta;NFKBIKB; Serine/threonine protein kinase IKBKB; IKBKB; IKKB

Immunogen: Synthetic peptide, corresponding to Human IKKβ.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: Autophagy Signaling,