IKKb Antibody| F40420-0.4ML/R31146

IKKb Antibody| F40420-0.4ML/R31146 | Gentaur UK, US & Europe Distribution

Background: NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).

Format: Purified

Clone: 62AT216

Host Animal: Mouse

Clonality: Monoclonal (mouse origin)

Isotype: Mouse IgG1

Species Reactivity: Human

Predicted Spacies Reactivity: N/A

Application: WB, ELISA

Application Details: Western blot: 1:100-1:500

Application Note: Titration of the IKKb antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Status: Available

Buffer: N/A

Limitation: This IKKb antibody is available for research use only.

Purity: Purified

Immunogen: This IKKb antibody was raised using purified His-tagged recombinant full length human IKK beta.

Storage: Aliquot the IKKb antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Localization: N/A