IL-10 Antibody [15B9-1-2] | 38-301

IL-10 Antibody [15B9-1-2] | 38-301 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Mouse

Homology: N/A

Immunogen: E.coli derived Recombinant Murine IL-10

Research Area: Infectious Disease, Neuroscience, Cancer, Stem Cell, Chemokines & Cytokines, Functional Assays

Tested Application: E, WB, Neut

Application: ELISA:
Sandwich:
In a sandwich ELISA (assuming 100μL/well) , a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 1600 pg/mL of recombinant murine IL-10 when used with ProSci’s Polyclonal biotinylated antigen affinity purified anti-murine IL-10 as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (0.5 ng/mL) , a concentration of 3.0-5.0 μg/mL of this antibody is required.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: This IgG2A antibody was purified from cell culture by Protein G affinity chromatography.

Clonality: Monoclonal

Clone: 15B9-1-2

Isotype: N/A

Conjugate: Unconjugated

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

Alternate Name: Interleukin 10Interleukin-10, Cytokine synthesis inhibitory factor, IL-10, CSIF, TGIF, GVHDS, IL10A

User Note: Centrifuge vial prior to opening.

BACKGROUND: N/A