IL-12A Antibody | 7497

IL-12A Antibody | 7497 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Llama: (94%) , Goat: (89%) , Pig: (89%) , Sheep: (89%) , Horse: (83%) , Bovine: (83%)

Immunogen: IL-12A antibody was raised against an 18 amino acid peptide near the carboxy terminus of human IL-12A.
The immunogen is located within the last 50 amino acids of IL-12A.

Research Area: Immunology, Innate Immunity

Tested Application: E, WB, IHC-P, IF

Application: IL-12A antibody can be used for detection of IL-12A by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: IL-12A antibody is human specific. IL-12A antibody will not cross-react with IL-12B.

Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 22 kDa
Observed: 22 kDa

Validation: N/A

Isoform: N/A

Purification: IL-12A antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: IL-12A antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: IL-12A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Interleukin 12 alpha, p35, natural killer cell stimulatory factor 1, NKSF1, cytotoxic lymphocyte maturation factor 1, CLMF, NF cell stimulatory factor chain 1, NFSK

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: IL-12A is subunit of the cytokine IL-12 which acts on T- and natural killer cells, and has a broad array of biological activities. IL-12 is a disulfide-linked heterodimer composed of the 35-kD subunit and a 40-kD subunit that is a member of the cytokine receptor family (1) . IL-12 is required for the T-cell-independent induction of interferon (IFN) -gamma, and is important for the differentiation of both Th1 and Th2 cells (1, 2) . The responses of lymphocytes to IL-12 are mediated by the activator of transcription protein STAT4 (2) . Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity (3) .