IL-17 Antibody | 4877

IL-17 Antibody | 4877 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: IL-17 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human IL-17A.
The immunogen is located within amino acids 40 - 90 of IL-17.

Research Area: Signal Transduction

Tested Application: E, WB

Application: WB: 0.125-4 μg/mL.
Antibody validated: Western Blot in human and mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1208 - THP-1 Cell Lysate

Positive Control 2: Cat. No. 1288 - A20 Cell Lysate

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 17.5kD
Observed: 20 kD (Post-modification: 1 N-linked glycosylation)

Validation:

Recombinant Protein Test (Figure 3) : Anit-IL-17 antibodies (4877) detected human IL-17 recombinant protein at different concentrations.

Isoform: Human IL-17 has only one isoform (155aa, 17.5kD) . Mouse IL-17 has one isoform (158aa, 17.5kD) and rat IL-17 also has one isoform (150aa, 16.9kD) . 4877 can detect only human isoform, but cannot detect mouse and rat.

Purification: IL-17 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: IL-17 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: IL-17 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: IL-17 Antibody: IL17, CTLA8, IL-17, IL-17A, IL17, Interleukin-17A, Cytotoxic T-lymphocyte-associated antigen 8

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: IL-17 Antibody: Interleukin 17 (IL-17) is a family of pro-inflammatory cytokines produced by activated T cells and is thought to have a major role in the initiation and perpetuation of rheumatoid arthritis. IL-17 regulates the activities of NF-κB and mitogen-activated protein kinases such as ERK and JNK. In addition, IL-17 stimulates the expression of IL-6 and cyclooxygenase-2 and enhances the production of nitric oxide. IL-17-producing T helper cells (TH-17 cells) have been the subject of much attention due to the importance of IL-17 in the pathogenesis of autoimmune inflammation. Because of its role in autoimmune diseases, it is thought that targeting the production and action of IL-17 would be beneficial therapeutically in these diseases.