IL-17A, human recombinant | 4176

The original described IL-17 protein, now known as IL-17A, was identified from a CD4+ T cells DNA library. IL-17 can be induced from primary peripheral blood CD4+ T cells upon stimulation. Supernatant from cells transfected with IL-17 induced IL-16 and IL-18 production and enhanced the surface expression of the intraCellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Recombinant IL-17 is a 31 kDa disulfide-linked homodier of two 136 amino acid polypeptide chains.

4176 | IL-17A human recombinant DataSheet

Biomolecule/Target: IL-17A

Synonyms: IL-17, CTLA-8

Alternates names: CA-II, CAC, Carbonic anhydrase 2, Carbonate dehydratase 2, can, cynT2, yadF, b0126, JW0122, Carbonic Anhydrase II.

Taglines: A proinflammatory cytokine produced by activated T cells

NCBI Gene ID #: 760

NCBI Gene Symbol: CA2

Gene Source: Human

Accession #: P00918

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: 98%

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: Specific activity is 50-70 nmoles/min/µg and was obtained by measuring the increase in the amount of p-nitrophenol by its esterase activity.

Results: 50-70 nmoles/min/µg

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 29.2 kDa (260 aa, 1-260 aa)

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered and lyophilized with no additives

Reconstitution Instructions: N/A

Amino acid sequence: MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL KNRQIKASFK