IL-1F10 Antibody | 7503

IL-1F10 Antibody | 7503 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Mouse (100%) and Rat (88%) .

Immunogen: Anti-IL-1F10 antibody (7503) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human IL-1F10.
The immunogen is located within the last 50 amino acids of IL-1F10.

Research Area: Immunology

Tested Application: E, WB, IHC-P

Application: WB: 1 μg/mL; IHC: 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples. All other applications and species not yet tested.

Specificiy: IL-1F10 antibody is human, mouse and rat reactive. Two isoforms of IL-1F10 exist.

Positive Control 1: Cat. No. 1304 - Human Liver Tissue Lysate

Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 17kD
Observed: 17 kD

Validation:

Recombinant Protein Test (Figure 1) : Anti- IL-1F10  antibodies (7503) detected human IL-1F10  recombinant protein at different concentrations.

Isoform: Human IL-1F10 has 2 isoforms, including isoform 1 (152aa, 17kD) and isoform 2 (152aa, 17kD) . Mouse IL-1F10 has one isoform (152aa, 17kD) and Rat IL-1F10 also has one isoform (152aa, 17kD) . 7503 can detect human, mouse and rat.

Purification: IL-1F10 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: IL-1F10 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: IL-1F10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: IL-1F10 Antibody: IL-38, FKSG75, IL1HY2, IL-1HY2, IL1-theta, FIL1-theta, FIL1T, IL38, UNQ6119/PRO20041, Interleukin-1 family member 10, Family of interleukin 1-theta, IL-1F10

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The cytokine IL-1 is responsible for initiating a variety of activities through the activation of transcription factors, NF-kappa B and AP-1, thereby promoting host response to injury or infection (1) . IL-1F10 (Interleukin-1 family member 10) , also known as FIL1T, IL1HY2 or FKSG75, is highly expressed in the skin, spleen and proliferating B-cells of the tonsil (2) . IL-1F10 binds soluble IL-1 receptor type 1, and is one of nine Interleukin 1 families clustered on chromosome 2, where it is thought to participate in the regulation of adapted and innate immune responses (3) . IL-1F10 and IL-1RN polymorphisms may play an important role in the susceptibility to developing rheumatoid arthritis (4) .