IL-2 Antibody [4N2Q9] | 11-089

IL-2 Antibody [4N2Q9] | 11-089 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Virus

Homology: N/A

Immunogen: Recombinant Human IL2 Protein, amino acids Ala21-Thr153

Research Area: Infectious Disease, COVID-19

Tested Application: E

Application: Human IL-2 Sanwich ELISA Immunoassay:

ELISA Capture: Recommended Concentration: 2-8ug/mL, Sample: Human IL-2 Monoclonal Antibody, Rabbit MAb (CAP)

ELISA Detection: Recommended Concentration: 0.15-0.5ug/mL, Sample: Human IL-2 Monoclonal Antibody, Rabbit Mab (DET)

Standard: Recommended Concentration: 0.78-50pg/mL, Sample: Recombinant Human IL2 Protein

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Affinity Purified, Endotoxin Level: < 1.0 EU/ug of the protein by LAL method.

Clonality: Recombinant Monoclonal

Clone: 4N2Q9

Isotype: Rabbit IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied as a 0.2um filtered solution in PBS, pH 7.4.

Concentration: 1mg/ml

Storage Condition: This antibody can be stored at 2˚C to -8˚C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20˚C to -80˚C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Alternate Name: IL-2; TCGF; lymphokine

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Interleukin-2 (IL-2) is a O-glycosylated, four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma �� T cells, B cells, dendritic cells, and eosinophils. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis. IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells.