IL-33 Antibody | 36-250

IL-33 Antibody | 36-250 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Mouse

Homology: N/A

Immunogen: Recombinant mouse IL-33.

Research Area: Innate Immunity

Tested Application: E, WB

Application: ELISA: (direct and indirect: 1:2, 000-1:5, 000) . Western Blot: (1:2, 000-1:5, 000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.

Specificiy: Recognizes mouse IL-33. Detects a band of ~18kDa by Western blot. Does not cross-react with human IL-33.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Concentration: 1 mg/ml

Storage Condition: Stable for at least 6 months after receipt when stored at -20˚C.

Alternate Name: Interleukin-33; IL-1F11; NF-HEV

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Interleukin-33 (IL-33; HF-NEV; IL-1F11) , a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kDa human IL33 is converted by CASP1 to a 18kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1 (MAPK3) /ERK2 (MAPK1) , p38 (MAPK14) , and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.