IL-3B Antibody (biotin) | XP-5187Bt

IL-3B Antibody (biotin) | XP-5187Bt | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Rat

Homology: N/A

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant rat IL-3-β (rat IL-3-β) .

Research Area: Immunology, Chemokines & Cytokines, Antibody Pairs

Tested Application: E, WB

Application: ELISA:
Sandwich:
To detect Rat IL-3β by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat IL-3β (XP-5187) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-3β.
Western Blot:
To detect IL-3-β by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant IL-3-β is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Anti-IL-3-β specific antibody was purified by affinity chromatography and then biotinylated.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Biotin

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: IL-3B antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

Alternate Name: N/A

User Note: Centrifuge vial prior to opening.

BACKGROUND: IL-3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells