IL-9 Antibody | 7495

IL-9 Antibody | 7495 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: N/A

Immunogen: Anti-IL-9 antibody (7495) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IL-9.
The immunogen is located within the last 50 amino acids of IL-9.

Research Area: Immunology

Tested Application: E, WB, IHC-P, IF

Application: WB: 1-4 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: IL-9 antibody is human specific. IL-9 antibody is predicted to no cross-react with IL-7.

Positive Control 1: Cat. No. 1306 - Human Spleen Tissue Lysate

Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 3: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 16kD
Observed: 22 kD (Post-modification: 4 N-linked glycosylations)

Validation:

Recombinant Protein Test (Figure 2) : Anti-IL-9 antibodies (7495) detected human IL-9 recombinant protein at different concentrations.

Isoform: Human IL-9 has one isoform (144aa, 16kD) . Mouse IL-9 has one isoform (144aa, 16kD) and Rat IL-9 also has one isoform (144aa, 16kD) . 7495 can detect human isoform, but cannot detect mouse and rat.

Purification: IL-9 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: IL-9 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: IL-9 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: IL-9 Antibody : P40, HP40, IL-9, Interleukin-9, Cytokine P40

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Interleukin 9 (IL-9) is a cytokine secreted by TH2 lymphocytes that acts as a regulator of a variety of hematopoietic cells, stimulates cell proliferation and prevents apoptosis (1, 2) . It functions through the interleukin 9 receptor (IL9R) , which activates different signal transducer and activator (STAT) proteins (3) . The IL-9 gene has been identified as a candidate gene for asthma (4) . Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness (5) .