Immortalized Baby Mouse Kidney Epithelial Cells (iBMK) | T0082

Immortalized Baby Mouse Kidney Epithelial Cells (iBMK) | T0082

Immortalization Method:

Serial passaging and electroporation transfection with linearized DNA of E1A and p53DD

BioSafety Level:

II

Organism:

5-day-old wild-type baby C57B/6 mouse

Species:

Mouse

Source Organ:

Kidney

Organ Type:

Renal

Growth Properties:

Adherent

Morphology:

Cuboidal

Passage Number:

5

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

E-cadherin, CK8 and CK18

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available from abm . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1. Western blot was used to confirm the presence of E1A and p53DD gene in the immortalized cell line. 2. Western blot was used to check epithelial markers E-cadherin and cytokeratin expression.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C