Immortalized Canine Bone Marrow Mesenchymal Stromal Cells (DS1) | T0687

Immortalized Canine Bone Marrow Mesenchymal Stromal Cells (DS1) | T0687

Immortalization Method:

Transduction with retrovirus containing the HPV E6/E7 genes

BioSafety Level:

II

Organism:

Canine

Species:

Canine

Source Organ:

Bone Marrow

Organ Type:

Skeletal Muscle

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

15 - 20 hours

Seeding Density:

20,000 cells/cm10²

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%, L-glutamine and Penicillin/Streptomycin Solution to a final concentration of 1%. Non-essential amino acid and sodium pyruvate may also be added.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C