Immortalized Human Adrenocortical Cells- hTERT | T0621

Immortalized Human Adrenocortical Cells- hTERT | T0621

Immortalization Method:

Serial passaging and transduction with recombinant retroviruses carrying hTERT gene

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Adrenal gland

Organ Type:

Renal

Growth Properties:

Adherent

Morphology:

Other

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%, 10% heat-inactivated horse serum, 0.1ng/ml recombinant Human FGF2 (Z101455) and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1. Telomerase repeat amplification protocol (TRAP) assay was performed to measure telomerase activity in the immortalized cell line. 2) RT-PCR was used to quantify telomerase RNA component (TERC) expression in the immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C