Immortalized Human Aortic Endothelial Cells- SV40 | T0447

Immortalized Human Aortic Endothelial Cells- SV40 | T0447

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Aorta

Organ Type:

Blood Vessel

Growth Properties:

Adherent

Morphology:

Epithelial (<10% fibroblasts)

Passage Number:

N/A

Population Doubling:

34 - 44 hours

Seeding Density:

10,000 - 30,000 cells/cm10²
* Note that cells are slow growing and may take up to 1 week to recover from thaw.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow I medium available at abm. to a final concentration of 10%, and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify the transgene expression.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C