Immortalized Human Bone Marrow Mesenchymal Cells - hTERT | T0523

Immortalized Human Bone Marrow Mesenchymal Cells - hTERT | T0523

Immortalization Method:

Serial passaging and transduction with recombinant VSV-G retrovirus carrying hTERT and GFP genes

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Bone Marrow

Organ Type:

Blood

Growth Properties:

Adherent

Morphology:

Spindle shaped

Passage Number:

N/A

Population Doubling:

35-45 hours

Seeding Density:

10,000 - 20,000 cells/cm10²; Recommended split ratio is 1:2 to 1:3

Markers:

CD44 and CD73. These cells also express markers of osteoblasts (alkaline phosphatise) and chondrocytes (incorporation of toluidine blue dye)

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm. to a final concentration of 10%, hydrocortisone to 10^-6 mol/L and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1. Immunofluorescence and RT-PCR were used to confirm the presence hTERT expression. 2. PCR based TRAP was used to assay telomerase activity. 3. Neo concentration not determined for selection use but cells were sorted for GFP+ populations

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C