Immortalized Human Bronchial Epithelial Cells (HBEC3-KT) - Cdk4/hTERT | T0753

Immortalized Human Bronchial Epithelial Cells (HBEC3-KT) - Cdk4/hTERT | T0753

Immortalization Method:

Immortalized with pSRαMSU expressing Cdk4 and pBABE expressing hTERT

BioSafety Level:

II

Organism:

Human

Species:

Human

Source Organ:

Lung

Organ Type:

Lung

Growth Properties:

Adherent

Morphology:

Epithelial

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

G418, puromycin

Donor Age:

N/A

Donor Gender:

Not disclosed

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow X medium available at abm. TM0753.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

1) PCR; 2) Immunocytochemistry; 3) Karyotyping; 4) Microarray

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C