Immortalized Human Nasal Epithelial Cells - SV40 | T0440

Immortalized Human Nasal Epithelial Cells - SV40 | T0440

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Nasal Mucosa

Organ Type:

Airway/Lung

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

40 - 50 hours

Seeding Density:

10,000 - 15,000 cells per cm10² (when thawing); recommended split ratio, post-thaw, of 1:2 (~25,000 cells/cm10²)

These cells must be split at a low ratio (1:2) for successful growth.

Markers:

Cytokeratin

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow X medium available at abm. TM0440.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm recommends using Accutase with T0440. This cell line is highly sensitive to trypsin.

Quality Control:

Real Time PCR was used to quantify SV40 gene expression in immortalized cell line. Tested negative for fungi and bacterial growth, mycoplasma, HIV-1, HBV, and HCV.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C