Immortalized Human Neuronal Progenitor Cells (Neu41) | T0781

Immortalized Human Neuronal Progenitor Cells (Neu41) | T0781

Immortalization Method:

Immortalized via introduction of cytoplasmic content (non-DNA) of tumor cells into primary cells

BioSafety Level:

II

Organism:

Human (H. sapiens)

Species:

Human

Source Organ:

Brain

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Neuronal

Passage Number:

N/A

Population Doubling:

24 - 30 hours

Seeding Density:

20,000 - 40,000 cells/cm²

Markers:

Beta III tubulin, MAP-2

Donor Age:

Not disclosed

Donor Gender:

Not disclosed

Donor Ethnicity:

Not disclosed

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%, Penicillin/Streptomycin Solution to a final concentration of 1%, Recombinant Human FGF2 to a final concentration of 10 ng/ml, Recombinant Human GH1 to a final concentration of 10 ng/ml, and Recombinant Human HGF to a final concentration of 10 ng/ml.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
To differentiate cells into neurons: Plate the cells on coated culture vessels at a density of 10⁴ cells/cm², in Prigrow IV containing 5% fetal bovine serum , 10 ng/ml Recombinant Human FGF2 , and 100 µM dibutyryl cAMP. The cells should be left in the differentiation medium for at least one week before testing for neuron specific markers.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) IHC

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C