171
Immortalized Human Skin Fibroblast - HPV E6/E7 | T0904
- SKU:
- 171-T0904-GEN
- Availability:
- IN STOCK
Description
Immortalized Human Skin Fibroblast - HPV E6/E7 | T0904
Immortalization Method:
Serial passaging and transduction with recombinant lentiviruses carrying HPV E6/E7 gene
BioSafety Level:
II
Organism:
Homo sapiens
Species:
Human
Source Organ:
Skin
Organ Type:
Skin
Growth Properties:
Adherent
Morphology:
Bipolar
Passage Number:
N/A
Population Doubling:
N/A
Seeding Density:
N/A
Markers:
Puromycin resistance
Donor Age:
N/A
Donor Gender:
N/A
Donor Ethnicity:
N/A
Propagation:
The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10%, Penicillin/Streptomycin Solution to a final concentration of 1%, and L-glutamine to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control:
Real Time PCR was used to quantify HPV E6 and E7 gene expression in immortalized cell line.
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C