Immortalized MEF Psen1/2^-/- Cells | T0819

Immortalized MEF Psen1/2^-/- Cells | T0819

-/- cells were derived from E8.5 embryos which were PSEN negative, and is neomycin and hygromycin-resistant. The cells were immortalized with SV40 large T antigen via transfection with the pMSSVLT plasmid, and were continuously grown in culture to established the immortalized cell line.

Immortalization Method:

Immortalized with pMSSVLT plasmid carrying SV40 large T antigen

BioSafety Level:

II

Organism:

Mouse (M. musculus)

Species:

Mouse

Source Organ:

Embryo

Organ Type:

Embryo

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

Psen1/2^-/- Neomycin (G418) and hygromycin-resistant

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm. to a final concentration of 10%, and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Recommend split ratio of 1:15 - 1:20 in new cell culture flask twice a week.

Quality Control:

N/A

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C