Immortalized Mouse Dendritic Cells (MutuDC1940) | T0528

Immortalized Mouse Dendritic Cells (MutuDC1940) | T0528

+ subset (CD11c^high,B220^-,DEC205⁺,CD24^high,CD11b^-) of conventional dendritic cells derived from the CD11c:SV40LgT transgenic mice.

The MutuDC1940 cell line retains response to TLR ligands such as CpG (TLR9-L) and PolyIC (TLR3-L) and to a lesser extent LPS (TLR4-L) stimulation by up-regulation of co-stimulatory molecules CD40, CD80 and CD86. In addition to responding to PAMP stimulation and producing Th1 cytokines such as IL-12, these cells are also capable of presenting antigen in the context of both MHC-I and MHC-II, including direct antigen presentation and cross-presentation of cell-associated antigens. Together with TLR3 knockout (Cat. No. T3034), TLR9 knockout (Cat. No. T3035) and Ifnar1 knockout (Cat. No. T3036) MutuDC, these dendritic cell lines provide a powerful tool in vaccine science and immunotherapy, particularly in strategizing target antigens to CD8α⁺ subset.

Immortalization Method:

Isolated from C57BL/6 transgenic mice carrying SV40 Large T oncogene

BioSafety Level:

II

Organism:

CD11c:SV40LgT-transgenic C57BL/6 mice

Species:

Mouse

Source Organ:

Spleen

Organ Type:

Spleen

Growth Properties:

Adherent

Morphology:

Small aggregates

Passage Number:

N/A

Population Doubling:

45 - 55 hours

Seeding Density:

20,000 – 60,000 cells/cm10².

Recommended split ratio: no greater than 1:3.

Markers:

CD11c, CD24, MHC-II+, DEC205, Clec9A, B220, CD11b, IRF4, IRF8, CD4

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is IMDM (1x) + Glutamax^TM (Gibco Ref: 31980-030). To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%, 1% of 7.5% Sodium Bicarbonate Solution, 50 µM β-mercaptoethanol, HEPES to a final concentration of 10 mM, and Penicillin/Streptomycin Solution to a final concentration of 1%. Filter the complete media at 0.22µm before use.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

1) Direct antigen presentation and cross-antigen presentation were evaluated by the MHC-I (SIINFEKL/OT-I ) and MHC-II (OVA323-339/OT-II) restricted systems; 3) proteome profile and surface markers assessed by RT-PCR; RT-PCR; 3) IL-12 cytokine secretion analyzed using ELISA ; 4) Response to PAMP stimulation evaluated by functional assays.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C