171
Immortalized Mouse Lung Endothelial Cells - PmT | T0094
- SKU:
- 171-T0094-GEN
- Availability:
- IN STOCK
Description
Immortalized Mouse Lung Endothelial Cells - PmT | T0094
Immortalization Method:
Immortalized by infecting with polyoma middle T (PmT) virus
BioSafety Level:
II
Organism:
Mouse
Species:
Mouse
Source Organ:
Lung
Organ Type:
Airway/Lung
Growth Properties:
Adherent
Morphology:
Endothelial
Passage Number:
N/A
Population Doubling:
N/A
Seeding Density:
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers:
N/A
Donor Age:
N/A
Donor Gender:
N/A
Donor Ethnicity:
N/A
Propagation:
This cell line requires coated cell culture vessels of collagen (final concentration of 30ug/ml; G422) and fibronectin (final concentration of 10ug/ml; TM059) in 0.1% gelatin (TM063). The base medium for this cell line is a 1:1 (recommend 400ml:400ml) ratio mix of Prigrow VI and Prigrow IV Series medium available at abm. , 10 ml Penicillin/Streptomycin Solution , and 20 ml L-glutamine . Filter the medium with 0.2 uM filter before adding fetal bovine serum which is first heat-inactivated before use to final concentration of 10% and 50 mg of endothelial mitogen (Endothelial Cell Growth Supplement) (Generon).
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Quality Control:
N/A
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C