Immortalized Mouse Lung Endothelial Cells - PmT | T0094

Immortalized Mouse Lung Endothelial Cells - PmT | T0094

Immortalization Method:

Immortalized by infecting with polyoma middle T (PmT) virus

BioSafety Level:

II

Organism:

Mouse

Species:

Mouse

Source Organ:

Lung

Organ Type:

Airway/Lung

Growth Properties:

Adherent

Morphology:

Endothelial

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

This cell line requires coated cell culture vessels of collagen (final concentration of 30ug/ml; G422) and fibronectin (final concentration of 10ug/ml; TM059) in 0.1% gelatin (TM063). The base medium for this cell line is a 1:1 (recommend 400ml:400ml) ratio mix of Prigrow VI and Prigrow IV Series medium available at abm. , 10 ml Penicillin/Streptomycin Solution , and 20 ml L-glutamine . Filter the medium with 0.2 uM filter before adding fetal bovine serum which is first heat-inactivated before use to final concentration of 10% and 50 mg of endothelial mitogen (Endothelial Cell Growth Supplement) (Generon).
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

N/A

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C