Immortalized Mouse Pancreas Epithelial Cells-Conditionally (IMPE) | T0156

Immortalized Mouse Pancreas Epithelial Cells-Conditionally (IMPE) | T0156

b promoter and a temperature-sensitive simian virus 40 large T antigen. The functional expression of the SV40 large T antigen is induced by culturing the cells in vitro in medium containing IFN γ at a temperature permissive (33°C). At a non-permissive temperature (37°C-39°C) or when cultured without IFN γ over 3 passages, the cells cease to proliferate. The IMPE has been shown to produce insulin after transfection with pdx1 and culture in the presence of glucagon-like peptide 1 (GLP1).

Immortalization Method:

Isolated from transgenic mouse carrying a temperature-sensitive simian virus 40 tumor antigen (tsA58)

BioSafety Level:

II

Organism:

H-2K^b-tsA58 transgenic mouse

Species:

Mouse

Source Organ:

Pancreas

Organ Type:

Digestive

Growth Properties:

Adherent

Morphology:

Cobble-stone

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Recommended split ratio of 1:3

Markers:

β-catenin, E-Cadherin, CK8, CK18, CK19

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%, 5 U/ml murine IFN-γ and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Western Blot and immunofluorescence were used to detect the presence of SV40 large T antigen; 2) Western Blot was utilized to detect the expression of epithelial markers E-Cadherin and β-catenin; 3) RT-PCR was used to verify the expression of CK8, CK18 and CK19.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C