Immortalized Mouse Small Intestinal Epithelial Cells-Conditionally (MSIE) | T0568

Immortalized Mouse Small Intestinal Epithelial Cells-Conditionally (MSIE) | T0568

b promoter and a temperature-sensitive simian virus 40 large T antigen. The functional expression of the SV40 large T antigen is induced by culturing the cells in vitro in medium containing IFN γ at a temperature permissive (33°C). At a non-permissive temperature (37°C-39°C), the cells cease to proliferate and shows a 2-3X differentiation rate as indicated by expression levels of the brush border-associated enzymes, alkaline phosphatase, dipeptidyl peptidase IV, and sucrase.

Immortalization Method:

Isolated from transgenic mouse carrying a temperature-sensitive simian virus 40 tumor antigen (tsA58)

BioSafety Level:

II

Organism:

18-day-old H-2K^b-tsA58 transgenic mouse

Species:

Mouse

Source Organ:

Small intestine

Organ Type:

Digestive

Growth Properties:

Adherent

Morphology:

Other

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Recommended split ratio of 1:3

Markers:

CK18

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%, 5 U/ml murine IFN-γ and Penicillin/Streptomycin Solution to a final concentration of 1%
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

1) Immunohistochemical staining was used to detect the presence of SV40 large T antigen and keratin 18; 2) Biochemical assays (i.e. method of Nagatsu et al and method of Dahlquist) were used to measure brush border-associated enzymes alkaline phosphatase, dipeptidyl peptidase IV and sucrase.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C