Immortalized Mouse TMJ Disc Fibroblast-Like Cells | T0773

Immortalized Mouse TMJ Disc Fibroblast-Like Cells | T0773

Immortalization Method:

Stable transfection with the vector pGNR145 expressing hTERT

BioSafety Level:

II

Organism:

Mus musculus

Species:

Mouse

Source Organ:

Cartilage

Organ Type:

Skeletal

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Passage Number:

N/A

Population Doubling:

20 - 30 hours

Seeding Density:

5,000 - 10,000 cells/cm10²

Markers:

FSP1

Donor Age:

12 weeks old

Donor Gender:

Female

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow VIII medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Western blot; 2) qRTPCR; 3) Immunofluorescence

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C