Immortalized Porcine Aortic Endothelial Cells (AOC) | T0448

Immortalized Porcine Aortic Endothelial Cells (AOC) | T0448

Immortalization Method:

Tranfection with pRNS-1 plasmid encoding the SV40 genome

BioSafety Level:

II

Organism:

Large White Swine

Species:

Pig

Source Organ:

Aorta

Organ Type:

Blood Vessel

Growth Properties:

Adherent

Morphology:

Cobble-stone

Passage Number:

N/A

Population Doubling:

50-60 hours

Seeding Density:

25,000-30,000 cells/cm10²; Recommended split ratio is 1:2 to 1:3

Markers:

CD29, CD31, CD41/61, CD80/86, CD46, SWC3, LAMP-1

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Flow cytometry was used to confirm the expression of endothelial cell markers such as CD29, CD31, CD41/61, CD80/86, CD46, SWC3, LAMP-1 antigen and MHC Class I antigens; 2) ⁵¹Cr release assay was used to analyse the susceptibility of AOC to xenogeneic cell-mediated cytotoxicity; 3) 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine (DiI)-labelled Ac-LDL was used to evaluate Ac-LDL update by the AOC.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C