Immortalized Rat Hippocampal Cells (H19-7) | T0359

Immortalized Rat Hippocampal Cells (H19-7) | T0359

Immortalization Method:

Immortalized via retroviral transduction of the tsA58 and U19tsa simian virus 40 large tumor antigen.

BioSafety Level:

II

Organism:

Rattus norvengicus

Species:

Rat

Source Organ:

Hippocampus

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Elongated

Passage Number:

N/A

Population Doubling:

22 - 31 hours

Seeding Density:

N/A

Markers:

NFP, MAP-2, GAP-43 post-differentiation

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium, available at abm. to a final concentration of 10%, L-glutamine , 0.2 mg/ml G418, and Penicillin/Streptomycin solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C.

Protocol and Media for Differentiation:
The base medium for this cell line is Prigrow III medium, available at abm. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 1%, 20 nM hydrocortisone, 0.3 nM triiodothyronine, 0.1 mM putrescine, 20 nM progesterone, 1 pM estradiol, 30 nM sodium selenite, transferrin at 1 μg/ml, and insulin at 5 μg/ml. On Day 3, add 10 nM phorbol 12,13-dibutyrate (PBt2), the differentiation agent, to the culture.
Carbon dioxide (CO₂): 5%, Temperature: 39.0°C (nonpermissive).

Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Immunostaining

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C