Immortalized Rat Hypothalamus Cells (RCHT-1) | T0692

Immortalized Rat Hypothalamus Cells (RCHT-1) | T0692

β-hydroxylase and the norepinephrine transporter, NET. The Immortalized Rat Hypothalamus Cells (RCHT-1) were derived from the hypothalamus of a 4-month-old Fisher 344 rat and were immortalized spontaneously in cell culture. When cultured in vitro, the cells exhibit adherent growth properties and tend to grow in monolayers. The Immortalized Rat Hypothalamus Cells (RCHT-1) are useful in the field of neurology. Applications of this cell line include the study of dopamine and its role in neurological diseases.

Immortalization Method:

Spontaneous immortalization

BioSafety Level:

II

Organism:

Normal Fisher 344 rat

Species:

Rat

Source Organ:

Hypothalamus

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

Immunofluorescent-positive markers for dopamine β-hydroxylase and the norepinephrine transporter, NET

Donor Age:

4 months old

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%, 2 mM L-glutamine , and 1% Penicillin/Streptomycin Solution .
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C