Immortalized Rat Retinal Precursor Cells (R28) | T0575

Immortalized Rat Retinal Precursor Cells (R28) | T0575

of R28 cells is published describing the presence and absence of 8799 genes and ESTs that may relevant for investigators with an interest in using this cell line for a particular molecular analysis.

Immortalization Method:

Serial passaging and transduction with retroviruses carrying 12S E1A gene

BioSafety Level:

II

Organism:

Postnatal Day 6 Sprague-Dawley Rat

Species:

Rat

Source Organ:

Retinal Tissue

Organ Type:

Eye

Growth Properties:

Adherent

Morphology:

Other

Passage Number:

N/A

Population Doubling:

39.5 hours

Seeding Density:

Recommended split ratio of 1:2 to 1:3

Markers:

IRBP, S-antigen

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is DMEM. To make the completed growth medium, add the following components to the base medium: fetal bovine serum to a final concentration and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

1) Western blotting and immunocytochemistry were used to confirm the E1A transgene expression 2) RT-PCR and immunocytochemistry were used to assess the expression of the photoreceptor specific marker S-antigen and interphotoreceptor retinoid binding protein (IRBP)

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C