Description
Insulin ELISA | 10-1113-01
A high quality ELISA for specific measurement of human insulin in serum, plasma or cell culture media.
- Calibrated against 1st International Reference Preparation 66/304 for human insulin
- No cross-reactivity to C-peptide or Proinsulin des(31-32) or split (32-33)
- Insignificant or no cross-reactivity to insulin analogs
- CE/IVD labeled on select markets
The Mercodia insulin assays have little or no cross-reactivity to insulin analogs or to rat or mouse insulin and comes with full cross-reactivity data.
The Mercodia Insulin ELISAs are the only ELISA assays to satisfy the total error requirements in the Insulin Standardization Program.
The Mercodia Insulin ELISAs are the only ELISA assays to satisfy the total error requirements in the Insulin Standardization Program.
Test characteristics
Samples: Serum, plasma, cell culture media
Sample volume: 25 µL
Assay range: 3 - 200 mU/L
Detection limit: ≤ 1 mU/L (0.043 µg/L)
Incubation: 1 h + 15 min
Detection method: absorbance
Specificity
The following cross-reactions have been tested:
C-peptide <0.01 %
Proinsulin <0.01 %
Proinsulin des (31-32) <0.5 %
Proinsulin split (32-33) <0.5 %
Proinsulin des (64-65) 98 %
Proinsulin split (65-66) 56 %
Insulin aspart 4 %
Insulin detemir n.d.
Insulin glargin 24 %
Insulin glulisine n.d.
Insulin lispro n.d.
IGF-I <0.02 %
IGF-II <0.02 %
Rat insulin 0.7 %
Mouse insulin 0.3 %
Porcine insulin 93 %
Ovine insulin 48 %
Bovine insulin 31 %
n.d.=not detected
Additional Information
Packing: |
1 x 96 wells |
Measurement range: |
3 - 200 mU/L (0.13 - 8.70 µg/L) |
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