Integrin beta 3 Antibody [Y2/51] | 33-627

Integrin beta 3 Antibody [Y2/51] | 33-627 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: Phytohemagglutinin (PHA) -stimulated peripheral blood cells were used as the immunogen for the CD61 antibody.

Research Area: Cancer, Immunology, Signal Transduction

Tested Application: WB, Flow, IF, IHC-P

Application: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
IHC (FFPE) : 1-2 ug/ml for 30 min at RT
Titration of the CD61 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G affinity

Clonality: Monoclonal

Clone: Y2/51

Isotype: IgG1, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: Integrin beta-3, Platelet membrane glycoprotein IIIa, GPIIIa, CD61, ITGB3, GP3A

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: Reacts with human CD61, also called Integrin beta 3 (GPIIIa, vitronectin receptor beta chain) . It associates with the alphaV-chain (CD51) to form vitronectin receptor, or with the alphaIIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61) . The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.