IQSEC1 Antibody | 8009

IQSEC1 Antibody | 8009 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: IQSEC1 antibody was raised against a 19 amino acid peptide near the amino terminus of human IQSEC1.
The immunogen is located within amino acids 60 - 110 of IQSEC1.

Research Area: Cancer, Cell Cycle

Tested Application: E, WB, ICC, IF

Application: IQSEC1 antibody can be used for detection of IQSEC1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry at 2 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: IQSEC1 antibody is human, mouse and rat reactive. At least four isoforms of IQSEC1 are known to exist. IQSEC1 antibody is predicted to not cross-react with IQSEC2.

Positive Control 1: Cat. No. 1288 - A20 Cell Lysate

Positive Control 2: Cat. No. 17-208 - A-20 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 106 kDa
Observed: 103 kDa

Validation: N/A

Isoform: N/A

Purification: IQSEC1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: IQSEC1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: IQSEC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: IQ motif and Sec7 domain 1, ADP-ribosylation factors guanine nucleotide-exchange protein 100, ARFGEP100, BRAG2, Brefeldin-resistant Arf-GEF 2 protein

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: ADP ribosylation factors (Arfs) are small GTP-binding proteins known for their role in vesicular transport, where they nucleate the assembly of coat protein complexes at sites of carrier vesicle formation. IQSEC1 (GEP100/BRAG2) , a guanine nucleotide exchange protein for ARF6, belongs to the BRAG family and contains one IQ domain, one PH domain and one SEC7 domain (1) . As a known activator of ARF6, IQSEC1 regulates cell surface levels of Beta1 integrin and its knockdown blocked tumour metastasis (2, 3) . IQSEC1 links EGFR signaling to ARF6 activation and may contribute to the cancer metastasis and malignancy (4) .