IRS-1 Antibody | 3521

IRS-1 Antibody | 3521 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Rat: (80%)

Immunogen: IRS-1 antibody was raised against a 16 amino acid synthetic peptide from near the center of human IRS-1.
The immunogen is located within amino acids 910 - 960 of IRS-1.

Research Area: Signal Transduction

Tested Application: E, WB, ICC, IF

Application: IRS-1antibody can be used for the detection of IRS-1 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1216 - PC-3 Cell Lysate

Positive Control 2: Cat. No. 1286 - P815 Cell Lysate

Positive Control 3: Cat. No. 17-206 - P815 Cell Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: IRS-1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: IRS-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: IRS-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: IRS-1 Antibody: HIRS-1, Insulin receptor substrate 1, IRS-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: IRS-1 Antibody: Following tyrosine phosphorylation, the insulin receptor substrate 1 and 2 (IRS-1 and IRS-2) can form a protein scaffolding for the assembly of a host of Src homology 2 (SH2) domain-containing proteins. IRS-1 tyrosine phosphorylation can occur through the activity of several cytokine and growth factor receptors such as interleukin (IL) -4, IL-9, interferon-gamma, in addition to the insulin and insulin-like growth factor 1 receptors. The scaffolding provided by IRS-1 and IRS-2 is necessary for insulin signal transduction across the cell membrane. IRS-1 tyrosine phosphorylation, and thus formation of the IRS scaffolding is inhibited by tumor necrosis factor (TNF) , and this inhibition can itself be blocked by rapamycin, an inhibitor of the mammalian target of rapamycin (TOR) . TNF activity could also be blocked by inhibition of the Akt kinase and the PTEN tumor suppressor, suggesting that TNF impairs insulin signaling through IRS-1 by activation of the TOR signaling pathway.